Indirect coomb test

Indirect Coomb test COOi sampling instruction and materials are currently being reviewed at the lab.

Indirect Coombs Test (Indirect Antiglobulin Test, IAT): this test is used to detect antibodies circulating freely in the blood serum that could potentially react with certain antigens on red blood cells. It is often performed to verify the compatibility of blood transfusions and to investigate the presence of antibodies to red blood cells during pregnancy, which is important for preventing hemolytic disease of the newborn. In this test, the patient's serum is mixed with compatible red blood cells known to carry specific antigens. Antiglobulin serum is then added to see if agglutination occurs.

The direct Coombs test detects antibodies already bound to red blood cells, while the indirect Coombs test detects antibodies circulating freely in the serum that could potentially bind to red blood cells.